Models & Assays
Capsaicin
Acute pain can be studied via application of capsaicin (topical, subcutaneous or intradermal) which activates TRPV1-positive nociceptive fibers and triggers substance P release. Pain responses can be characterized with an array of behavioural tests such as von Frey, Hargreaves, hot/cold plate, paw pressure and tail flick assays. Capsaicin-induced hyperalgesia is responsive to morphine and other analgesic treatments.
In addition, capsaicin application onto the skin triggers a CGRP-mediated increase in dermal blood flow which can be quantified with laser Doppler perfusion imaging. This is a robust tool to screen effective CGRP-blocking treatments for migraine.
