Models & Assays
Light-Dark Dark Challenge Assay with Thigmotaxis
This multifaceted assay is pivotal for gauging the impact of potential anxiolytic compounds, providing a holistic view of zebrafish behavioural patterns. The assay evaluates the anxiety-like behaviour in zebrafish in a two-fold approach: first, measuring activity in response to light changes, and second, focusing on the zebrafish’s inherent tendency to stay close to walls or boundaries through thigmotaxis. Our research suggests that 10 μM diazepam demonstrates prominent anxiolytic effects.