TPA-Induced Dermatitis Model

TPA (12-O-tetradecanoylphorbol-13-acetate) applied topically to a mouse ear causes a strong local inflammatory response. It activates protein kinase C (PKC), driving inflammatory mediators and leukocyte recruitment, increased vascular permeability, and ultimately ear edema/swelling and erythema.

Left ear punch weight and left ear thickness at 24 hours
Bar chart of left ear punch weight (g) for five groups: vehicle/vehicle, TPA 0.1%/vehicle, TPA 0.1%/clobetasol, TPA 0.1%/indomethacin and TPA 0.1%/crisaborole, with significance brackets between groups. Bar chart of left ear thickness (mm) at 24 hours for the same five groups, with significance brackets between groups.

Vehicle/vehicle, TPA 0.1%/vehicle, TPA 0.1%/clobetasol, TPA 0.1%/indomethacin and TPA 0.1%/crisaborole groups.